Hypervariations of a protease-encoding gene, PD0218 (pspB), in Xylella fastidiosa strains causing almond leaf scorch and Pierce's disease in California.
نویسندگان
چکیده
Xylella fastidiosa is a gram-negative plant pathogenic bacterium that causes almond leaf scorch disease (ALSD) and Pierce's disease (PD) of grape in many regions of North America and Mexico. Of the two 16S rRNA gene genotypes described in California, A genotype strains cause ALSD only and G genotype strains cause both PD and ALSD. While G genotype strains cause two different diseases, little is known about their genetic variation. In this study, we identified a putative protease locus, PD0218 (pspB), in the genome of X. fastidiosa and evaluated the variation at this locus in X. fastidiosa populations. PD0218 contains tandem repeats of ACDCCA, translated to threonine and proline (TP), upstream of the putative protease conserved domain. Among 116 X. fastidiosa ALSD and PD strains isolated from seven locations in California, tandem repeat numbers (TRNs) varied from 9 to 47, with a total of 30 TRN genotypes, indicating that X. fastidiosa possesses an active mechanism for contracting and expanding tandem repeats at this locus. Significant TRN variation was found among PD strains (mean = 29.9), which could be further divided into two TRN groups: PD-G(small) (mean = 17.3) and PD-G(large) (mean = 44.3). Less variation was found in ALSD strains (mean = 21.7). The variation was even smaller after ALSD strains were subdivided into the A and G genotypes (mean = 13.3, for the G genotype; mean = 27.1, for the A genotype). Genetic variation at the PD0218 locus is potentially useful for sensitive discrimination of X. fastidiosa strains. However, TRN stability, variation range, and correlation to phenotypes should be evaluated in epidemiological applications such as pathotype identification and delineation of pathogen origin.
منابع مشابه
Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.
A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, ...
متن کاملWhole genome sequences of two Xylella fastidiosa strains (M12 and M23) causing almond leaf scorch disease in California.
Xylella fastidiosa is a Gram-negative plant-pathogenic bacterium causing many economically important diseases, including almond leaf scorch disease (ALSD) in California. Genome information greatly facilitates research on this nutritionally fastidious organism. Here we report the complete genome sequences of two ALSD strains of this bacterium, M12 and M23.
متن کاملBacteria and phytoplasmas / Bactéries et phytoplasmes Colony morphology of Xylella fastidiosa almond leaf scorch strains
Xylella fastidiosa is the causal agent of almond leaf scorch disease (ALSD), which is currently reemerging in California as a potential threat to almond (Prunus dulcis) production. We previously reported the presence of different colony morphotypes of X. fastidiosa ALSD strains on periwinkle wilt medium solidified with Gelrite and their association with genotypes or pathotypes after a low numbe...
متن کاملTwo Xylella fastidiosa Genotypes Associated with Almond Leaf Scorch Disease on the Same Location in California.
ABSTRACT Almond leaf scorch disease (ALSD) has recently reemerged in the San Joaquin Valley of California threatening almond production. ALSD is caused by Xylella fastidiosa, a nutritionally fastidious bacterium. Single nucleotide polymorphisms (SNPs) in the 16S rRNA gene (16S rDNA) of X. fastidiosa strains were identified to characterize the bacterial population in infected trees. Genotype-spe...
متن کاملMultilocus sequence typing of Xylella fastidiosa causing Pierce's disease and oleander leaf scorch in the United States.
Using a modified multilocus sequence typing (MLST) scheme for the bacterial plant pathogen Xylella fastidiosa based on the same seven housekeeping genes employed in a previously published MLST, we studied the genetic diversity of two subspecies, X. fastidiosa subsp. fastidiosa and X. fastidiosa subsp. sandyi, which cause Pierce's disease and oleander leaf scorch, respectively. Typing of 85 U.S....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied and environmental microbiology
دوره 74 12 شماره
صفحات -
تاریخ انتشار 2008